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micropipette puller  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company micropipette puller
    Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pipette+puller/Pipette+Holder+Assembly+(specify+%2C+or/pmc12908047-66-23-25
    Average 94 stars, based on 16 article reviews
    micropipette puller - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Injection:

    Article Title: Co-opted and canonical glycerol channels play a major role during anhydrobiosis of an extremophile crustacean
    Article Snippet: .. Females were placed in a 3% agarose cold plate for immobilization and the selected dsRNAs were injected into the ovisac [ ] employing a digital microinjector (XenoWorks, Sutter Instrument Co.) and a micropipette (Narishige, #GD-1) prepared with a Pipette Puller (Sutter Instrument Co.). .. To reduce the transcription of a single gene, females were injected with 200 nl of water with 1% phenol red (Merck, #P3532) containing 100 ng of the respective dsRNA, whereas for double depletion females were injected with 50 ng of each dsRNA in the same 200-nl volume.

    Transferring:

    Article Title: Co-opted and canonical glycerol channels play a major role during anhydrobiosis of an extremophile crustacean
    Article Snippet: .. Females were placed in a 3% agarose cold plate for immobilization and the selected dsRNAs were injected into the ovisac [ ] employing a digital microinjector (XenoWorks, Sutter Instrument Co.) and a micropipette (Narishige, #GD-1) prepared with a Pipette Puller (Sutter Instrument Co.). .. To reduce the transcription of a single gene, females were injected with 200 nl of water with 1% phenol red (Merck, #P3532) containing 100 ng of the respective dsRNA, whereas for double depletion females were injected with 50 ng of each dsRNA in the same 200-nl volume.

    Article Title: Purinergic P2Y 1 and P2Y 12 receptors control enteric nervous system activity through neuro-glia-macrophage crosstalk.
    Article Snippet: .. Glass micropipettes were fabricated from capillary glass (0.85 mm ID/ 1.25 mm OD, end-opening 60–80 μm of diameter) using a pipette puller (P-87 Flaming-Brown Micropipette Puller, Sutter Instruments Corporation) and were back-filled with ADP dissolved in Kreb’s Buffer. ..

    Article Title: mPFC DCC coupling with CaMKII + neuronal excitation participates in behavioral despair in male mice.
    Article Snippet: .. Patch electrodes (4–5MΩ) were pulled with a pipette puller (P2000; Sutter Instrument, USA) from borosilicate glass capillaries. ..

    Article Title: Single-Step Microfluidics-Based Method for Fabrication of Nanoparticle-Coated Functional Microfibers.
    Article Snippet: Fibers coated with nanoparticles have diverse applications as catalysts, biosensors, tissue scaffolds, and adsorbents for contaminants in water purification.. However, current fabrication techniques employ multistep processes that often result in uneven nanoparticle coatings, consequently leading to reduced activity.. Here we present a single-step microfluidics-based approach for the generation of microfibers with uniformly coated nanoparticles.

    Article Title: Structural and functional mechanisms of anti-NMDAR autoimmune encephalitis.
    Article Snippet: Autoantibodies against neuronal membrane proteins can manifest in autoimmune encephalitis, inducing seizures, cognitive dysfunction and psychosis.. Anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis is the most dominant autoimmune encephalitis; however, insights into how autoantibodies recognize and alter receptor functions remain limited.. Here we determined structures of human and rat NMDARs bound to three distinct patient-derived antibodies using single-particle electron cryo-microscopy.

    Article Title: mPFC DCC coupling with CaMKII + neuronal excitation participates in behavioral despair in male mice
    Article Snippet: .. Patch electrodes (4–5 MΩ) were pulled with a pipette puller (P2000; Sutter Instrument, USA) from borosilicate glass capillaries. ..

    Article Title: Sleep-wake-related changes in intracellular chloride regulate plasticity at glutamatergic cortical synapses.
    Article Snippet: On an experiment day, stock solution was added to the aCSF to achieve the desired final concentration (in mM): 10 bumetanide (NKCCI inhibitor, Tocris), 10 VU0463271 (KCC2 inhibitor, Tocris). .. Patch pipettes (2-5 MOhm) were pulled from standard wall borosilicate glass capillaries (Warner Instruments) with a custom-made program on a pipette puller (Sutter Instrument). .. Neurons were visualized under a 60x water-immersion objective (Olympus BX51WI).



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